Ali Taheri Mirghaed - Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Melika Ghelichpour - Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Abbasali Aghaei Moghaddam - Center for International Scientific Studies & Collaboration (CISSC), Ministry of Science Research and Technology, Tehran, Iran.
Seyed Hossein Hoseinifar - Center for International Scientific Studies & Collaboration (CISSC), Ministry of Science Research and Technology, Tehran, Iran.
Seyyed Morteza Hoseini - Center for International Scientific Studies & Collaboration (CISSC), Ministry of Science Research and Technology, Tehran, Iran.

Background: Organic acids and their salts are known as appropriate substitutes in feed for improving the health, growth, and performance of fish. Objectives: The present study was conducted to determine the effects of dietary lactic acid supplementation on immunological factors, hepatic enzyme activity, and plasma proteins in common carp, Cyprinus carpio. The fish were fed the diets mentioned above for ۵۶ days, then their growth performance, humoral immunity, and plasma hepatic enzymes were assessed.Methods: A total of ۱۸۰ fish (mean weight=۲۵ g) were randomly distributed in twelve tanks (۱۵۰ L water in each tank) as four treatments, fed diets containing ۰, ۲.۵, ۵, and ۱۰ g/kg lactic acid (T۰, T۱, T۲, and T۳, respectively).Results: At the end of the feeding trial, T۲ showed significantly higher growth performance than T۰. Plasma total protein, albumin, and globulin levels of T۱-T۳ were significantly higher than that of T۰. However, plasma protein levels decreased significantly by elevation in dietary lactic acid concentration (۱۰ g/kg). No significant differences were observed in plasma aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activity among the treatments, although alanine aminotransferase (ALT) activity decreased significantly in fish-fed dietary lactic acid supplementation (T۱-T۳) compared with the control group (T۰). All humoral immunity parameters (lysozyme, complement, immunoglobulin, and bactericidal activity) increased significantly in T۱-T۳ treatments compared to the T۰ group.Conclusion: Overall, dietary lactic acid supplementation improves growth performance, humoral immunological parameters, and hepatic health. According to the results, dietary lactic acid (۲.۵-۵ g/kg) is recommended for preparing common carp feed.

Food additives, Common carp, hepatic health, immunity status, lactic acid