In vitro Studies of Polycaprolactone Nanofibrous Scaffolds Containing Novel Gehlenite Nanoparticles
Publish place: Journal of medical signals and sensors، Vol: 11، Issue: 2
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JMSI-11-2_007
تاریخ نمایه سازی: 28 تیر 1402
Abstract:
Background: Recently, many studies have been done on the physicochemical properties and
biocompatibility of polycaprolactone (PCL) scaffolds containing ceramic reinforcers in the field
of bone tissue engineering. In this study, the physical, mechanical and biological properties of
electrospined‑fabricated PCL scaffolds containing gehlenite (GLN) nanoparticles (NPs) as a novel
bioceramic were investigated. Methods: To obtain the appropriate mechanical properties, the solution
contains ۳%, ۵%, ۷%, and ۱۰% wt. of GLN NPs were prepared. Fiber morphology was investigated
by scanning electron microscopy. In order to evaluate the NPs distribution, Energy Dispersive X‑Ray
Spectroscopy, X‑ray diffraction, and Fourier Transform Infrared Spectroscopy spectroscopy were used.
The scaffold hydrophilicity was measured by the water contact angle test. The tensile test was used to
check the mechanical strength of the scaffold. The proliferation of MG‑۶۳ cells was evaluated by the
MTT test. Alkaline phosphatase (ALP) activity of MG‑۶۳ cells was also examined. Results: Average
fibers’ diameters and porosity of PCL/GLN۷% were obtained ۱۵۰–۵۰۰ nm and ۸۰%, respectively. An
increase in the scaffold hydrophilicity was observed by the addition of GLN NPs. The strength of
PCL/GLN۷% was higher than the blank PCL scaffold. Cell proliferation of scaffolds containing GLN
was higher than the blank PCL scaffold. A significant increase in the secretion of ALP for GLN‑loaded
scaffolds was seen. Discussion: The results showed that PCL/GLN۷% composite scaffold could
be a good candidate for bone tissue engineering. Conclusion: The overall results indicate that the
scaffold (PCL /GLN۷%) has suitable mechanical properties, a great cell compatibility for bone tissue
regeneration.
Keywords:
Authors
Moloud Amini Baghbadorani
Student Research Committee, School of Advanced Technology in Medicine, Isfahan University of Medical Sciences
Ashkan Bigham
Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences
Mohammad Rafienia
Biosensor Research Center, Isfahan University of Medical Sciences
Hossein Salehi
Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran