Nastaran Barati - Hamadan university of medical sciences
Seyed Mousa Motavalli haghi - Hamadan university of medical sciences

Worm parasites, including Echinococcus granulosus, contain compounds that exhibit antitumor activity. So, the objective of this study was to evaluate the effects of antigen B extracted from hydatid cyst fluid on liver Hep G۲Methods: Effects of different concentrations of antigen B on Hep G۲ and HEK۲۹۳ cells proliferation were investigated using the MTT assay. Cell cycle analysis to measure cellular DNA content in the G۰/G۱, S and G۲/M was done using Flow cytometry. Annexin-V/PI staining method was used to determine cells apoptotic rate. Furthermore, the mRNA expression of pro-apoptotic gene BAX and anti-apoptotic gene BCL۲ was assessed by RT-PCR after exposure to antigen B.Results: The effect of antigen B on HEK۲۹۳ and Hep G۲ cells showed that HEK۲۹۳ cells as a normal cell line is less sensitive than cancer cell line B۱۶F۱۰ to antigen B, and IC۵۰ values in HEK۲۹۳ and Hep G۲cells were ۳۵ ± ۴.۳ and ۱۵ ± ۳.۱ μM, respectively. In both cell lines, the antigen B induced anti-proliferative effect on the cells with increasing cell population at G۰/G۱, and decreasing the numbers of cells at the S and G۲/M phases. Our results also showed antigen B can induce cell apoptosis in both HEK۲۹۳ and B۱۶F۱۰ cell lines and increased the mRNA expression of BAX and decreased the mRNA expression of BCL۲ thereby induction of cell apoptosis.Conclusion: this study confirmed that antigen B inhibits proliferation and promotes apoptosis of HEK۲۹۳ and Hep G۲cells and can raise hopes in the treatment of melanoma cancesupplement in the toxicity caused by rice tablets.