وحید چالشی - Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
سعیده علومی کلانتر - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
ایمان سلحشوری فر - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
شبنم شاهرخ - Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
حمید اسدزاده - Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
محمد رضا زالی - Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Background & Objective: Inflammatory bowel disease (IBD) is characterized by the chronic gastrointestinal inflammation. The two common forms of IBD are ulcerative colitis (UC) and Crohn’s disease (CD) that are distinguished by their location and depth of involvement in the diffuse inflammation of the colonic mucosa and affects the rectum (proctitis). A novel class of LncRNAs transcribed from ultra-conserved regions (UCRs) is a recently identified ultra-conserved region (T-UCR) transcript that is involved in the cellular function in a variety of pathways. However, the regulation of LncRNA uc.۱۷۳ in IBD remains to be fully elucidated. In this study, we aimed to examine the expression of LncRNA uc.۱۷۳ and Occludin genes in an Iranian population with inflammatory bowel disease. Materials & Methods: This case-control study was performed on ۳۳ inflammatory bowel disease patients including ۱۳ Crohn's disease, ۲۰ ulcerative colitis and ۲۰ healthy controls. The mRNA levels of LncRNA uc.۱۷۳ and Occludin genes were assessed using the quantitative Real-time polymerase chain reaction. The B۲M was used as an internal control. The ۲ -ΔΔCq method was used to determine the expression fold changes. Results: Statistically, the level of the LncRNA uc.۱۷۳ gene expression between the UC and normal tissues increased significantly(P=۰.۰۰۲۴). Also, the expression analysis revealed no significant difference between the samples of CD patients compared to the controls (P>۰.۰۵). In order to further evaluate the role of LncRNA uc.۱۷۳ in IBD, the associations between the transcript levels of the LncRNA uc.۱۷۳ and Occludin mRNA demonstrated significant difference in the CD tissue (R=۰.۵۹; P=۰.۰۰۲). In our study, the mRNA expression of Occludin gene did not show any changes in the IBD patients compared to the healthy controls. Conclusion: The increased expression of LncRNA uc.۱۷۳ in the tissues of UC patients may be considered as a diagnostic or prognostic biomarker. Also, there was no correlation found between Occludin and LncRNA uc.۱۷۳ expressions in the IBD patients' tissues.

Inflammatory bowel disease, Ulcerative colitis, Crohn's disease, LncRNA uc.۱۷۳, Occludin