غلامعباس محمدی - Department of Biochemistry, Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
حسن عزیزی - Department of Biochemistry, Kerman University of Medical Sciences, Kerman, Iran.

Background and Aims: Type and saturation of fatty acids can have an important impact on the level of triglyceride, cholesterol, very low and low-density lipoproteins in the blood and thus affect the development of atherosclerosis. Saturated fatty acids have an additive effect on blood cholesterol while for unsaturated fatty acids, a lowering effect has been reported. Fatty acids can have different effects on lipoproteins metabolism and apolipoproteins expression because oils used by human have different compositions. One of the important apolipoprotein is apolipoproteinB ( apoB) .This study was conducted to compare the effect of different nutritious fats on expression of apoB protein. Materials and Methods: For this purpose, ۴۸ Syrian male mice were selected and randomly divided into six groups of eight: chow, diet with۱۰%corn oil, diet with۱۰% olive oil, diet with۱۰% cow ghee), diet with۱۰% sheep ghee, and diet with ۲% cholesterol. After two months, liver and intestine were removed and transferred into liquid nitrogen and were frozen at -۷۰ºC. Protein was extracted and the expression of apoB was studied by western blotting. Results: An increase in the expression of intestinal apoB۴۸ was identified in olive oil and cow ghee groups. Hepatic apoB۱۰۰ expression increased in the cholesterol group compared with the corn oil group. Conclusions: This study indicates that olive oil and cow ghee consumption increase intestinal apoB۴۸ expression.

ApoB, Cholesterol, Corn oil, Cow ghee, Olive oil, Sheep ghee, آپولیپوپروتئین B, روغن گیاهی, روغن جانوری, کلسترول