منیژه آذری - Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
جواد محیطی اردکانی - Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
سارا عابدینی - Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
محمد رضا مزین - English Language Department, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Background and Aims: Ferula Assafoetida is an antioxidant plant which has long been used in Iranian traditional medicine. Recently, it has been reported to have hypoglycemic and hyperinsulinemic effects, but the molecular mechanism of this effect have not been sufficiently described. This study was a step to evaluate the molecular mechanism of Ferula assafoetida action as an antihyperglycemic agent. For this purpose, some signling pathways of the hypoglycemic effect of its extract using C۲C۱۲ mouse cell line were examined. Materials and Methods: C۲C۱۲ cells were differentiated in DMEM medium supplemented with ۲% heat inactivated horse serum, and treated with ۱۰ µg/ml extract of assafoetida in presence or absence of phosphoinositide ۳-kinase (PI-۳K) inhibitor. The concentration of Glucose transporter type ۴ (GLUT۴) in cytosol and cytoplasmic membrane were determined using SDS–polyacrylamide gel electrophoresis and western blotting analyses. Results: Data indicated that assafoetida treatment increases translocation of the GLUT۴ to the cell membrane in C۲C۱۲ cell line via PI۳K/Akt signaling pathway activation. Conclusions: our finding indicated that assafoetida has a potential antidiabetic effect and may be considered as antidiabetic drug.

C۲C۱۲ cell line, Diabetes, Ferula Assafoetida, Hypoglycemic Effect, رده سلولی C۲C۱۲, دیابت, انغوزه