Fahimeh Rahmani - Department of Agronomy and Plant Breeding, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Roya Rezaeian-Doloei - Department of Agronomy and Plant Breeding, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Leila Alimoradi - Department of Agronomy and Plant Breeding, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Background and Aims: Mentha pulegium L. is one of the medicinal plants used in the food production as a flavoring substance and its antibacterial effect has also been considered. The present study was designed to evaluate the phytochemical composition of Mentha pulegium L. originated from Iran and its antimicrobial activity against several pathogenic bacteria. Materials and Methods: The chemical compounds of this medicinal plant were analyzed by GC-MS and ۱۴ components were identified, representing ۸۶.۹۸% of its total essential mass. The antibacterial activity of the essential oil (EO) of Mentha pulegium L., has been evaluated against Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, and Pseudomonas syringae pv. tomato using disc diffusion method compared to synthetic antibiotic ciprofloxacin. The minimum inhibitory concentration (MIC) was evaluated against the above tested bacteria using macrobroth dilution method. Results: Results showed that the EO of Mentha pulegium L., was formed by the main components of piperitone (۳۲.۱%), piperitenone (۲۱.۷۱%) and pulegone (۱۵.۸۵%). In addition, the results obtained using MIC and MBC showed that the minimum inhibitory concentration of the EO of M. pulegium was in the range of ۱۰-۴۰ mg/mL for S. aureus, E. coli, P. aeroginosa and ۲۰ mg/mL for Ps. syringae pv. tomato, respectively. According to the results, the maximum zones of inhibition (۲۹.۱۵ mm) was observed on P. syringae P. tomato in ۴۰ mg/mL of essential oil, and the minimum zone of inhibition (۱۷.۰۸ mm) was related to P. aeroginosa in the same concentration. Conclusions: The results revealed that EO of Mentha pulegium L. had an appropriate inhibitory effect on Staphylococcus aureus and Escherichia coli.

Antibacterial activity, Mentha pulegium, Essential oil, MIC, MBC, ضد باکتریایی, پونه, اسانس گیاهی, MIC, MBC