Reza Ghotaslou - Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran.
Hojatollah Saghati - Departments of Microbiology, Tabriz University of Medical Sciences, Tabriz, Iran
Alireza Dehnad - High School of Rab- Rashid, Tabriz Iran
Behnaz Salahi Eshlaghi - Research center of Midwife School, Tabriz University of Medical Sciences, Tabriz, Iran

Background and Aim: Pseudomonas aeruginosa is an opportunist pathogen.  The infections due to biofilm are difficult to eradicate with current antimicrobial agents. In this study, we investigated the antimicrobial and antibiofilm activity of the honey against ۴۰ Pseudomonas aeruginosa isolates.  Materials and Methods:  To assess antimicrobial activity, the MIC and MBC assays were used. Antibiofilm activity of tested honey evaluated using the MTP, Congo Red Agar, and tube methods. Results: The initial screening demonstrated that ۴۰ of ۸۰ Pseudomonas aeruginosa isolates were biofilm- producer. The honey exhibited a significant antibacterial activity against the planctoninc form of Pseudomonas aeruginsa.  The MIC of Polak, Baboneh and Yonjeh honeys against Pseudomonas aeruginsa were ۶.۲۵-۲۵, ۱۲.۵-۲۵, and ۱۲.۵-۲۵% w/v, respectively. The results indicated that the tested honey also had significant antibiofilm activity. Conclusion: Antibacterial effects of honey are observed in both planctonic and biofilm forms. However, higher concentrations of honey have needed to inhibit biofilm. These findings indicate the potential antimicrobial of Azerbaijan honey and, it can be used in the treatment of bacterial infections diseases due to Pseudomonas aeruginosa.

Honey, Biofilm, Pseudomonas aeruginosa, بیوفیلم, عسل, پسودوموناس ائروژینوز