Extraction, identification and isolation of harmaline from Peganum hamala L. by HPLC chromatograghy

Several alkaloids that function as monoamine oxidase inhibitors (MAOIs) are found in the seeds of Peganum harmala (also known as Harmal or Syrian Rue), including harmine, harmaline,and harmalol, which are members of a group of substances with a similar chemical structure collectively known as harmala alkaloids Many species have been found to contain beta-carbolineharmala alkaloids.[8][9] which are MAO inhibitors with anti-depressant properties. The basis for the extraction of harmala alkaloids was the acidification of the harmalas followed by the removal of impurities with organic solvent , and neutralization followed by the removal of the harmala into ethyl acetate. in this case to reach a higher yield of them, bicarbonate are applied instead of chloroform as an organic phase. To purify harmaline , the crude extract is isolated by HPLC method. .Ectraction was studied by infrared chromatograghy. harmaline was separated using a Metasil ODS column by isocratic elution that the mobile phase is potassium phosphate buffer with flow rate 1.5 ml/min. From 1.2 g of the crude extract, 554 mg harmine and 325 mg harmaline were obtained each with a purity of over 96% as determined by HPLC.structure Identification of fractions was carried out by 1H NMR, and 13C NMR analyses. detection system is UV-visible spectroscopy (λ:330nm).

Keywords:

beta-carboline , harmaline , harmine , isolation , HPLC