Nadia Muslim - Department of Chemistry, Al-Najaf Education Directorate, Ministry of Education, Iraq
Barakat Hussain - Department of Chemistry, Al-Rsaffa Al-Ulla Directorate, Ministry of Education, Baghdad, Iraq
Noor Abdulhussein - Department of Chemistry, Al-Qadisiyah Education Directorate, Ministry of Education, Iraq
Ebaa Azooz - Medical Laboratory Technology Department, College of Medical Technology, The Islamic University, Najaf, Iraq

For the first time, air-assisted cloud point extraction (AA-CPE) procedure was applied to determine selenium in black tea samples at room temperature. The Air agitation is powered by a small fish tank generator to improve the dispersion phase and reduce the critical temperature for surfactants. The ligand and surfactant were ۱-(۲-hydroxy-۵-ptolylazo-phenyl)-ethan-one (HPAPEO) and TritonX-۱۰۰, respectively. Afterwards, the extracted selenium complexes were injected into a hydride generation atomic absorption spectrometer (HG-AAS). At the optimal conditions of experimentation, a linearity from ۰.۳ to ۱۰۰۰ µg L-۱ was constructed with an enhancement factor, preconcentration factor, detection limit, and extraction recovery of ۱۵۰, ۱۰۰, ۰.۰۲ µg L-۱, and ۹۹.۱–۱۰۲%, respectively. The AA-CPE technique's accuracy has been proven using certified reference materials. Ultimately, the Red-Green-Blue (RGB۱۲), Analytical Greenness Metric Approach (AGREE), and Complementary Green Analytical Procedure Index (ComplexGAPI) scales have been applied to assess the whiteness and greenness of this method. An accurate evaluation of the criteria has been performed to ensure this procedure is eco-friendly for the environment.

Air-assisted cloud point extraction, Analytical greenness metric approach, Complementary green analytical procedure index, Whiteness scale, selenium, Tea Leaves