Alwin Beschi Durairaj - Department of Botany & Research Center, Scott Christian College, Nagercoil – ۶۲۹۰۰۳, Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli – ۶۲۷۰۱۲, Tamil Nadu, India
Ananthi Sivaganam - Department of Proteomics, Clinbiocare Technology, Tenkasi – ۶۲۷ ۸۱۴, Tamil Nadu, India
Reginald Appavoo Monikam - Department of Botany & Research Center, Scott Christian College, Nagercoil – ۶۲۹۰۰۳, Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli – ۶۲۷۰۱۲, Tamil Nadu, India
Rajapandiyan Krishnamoorthy - Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh ۱۱۴۵۱, Kingdom of Saudi Arabia
Mohammad Z. Ahmed - Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
Alqahtani Ali S. - Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
Ponani Kaja Mideen - Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi, USA

Objective(s): The present study evaluated the protein-based analysis to unravel the role and mechanism behind the Dendropthae falcata plant extract treatment in breast cancer cells. Materials and Methods: The protein sample was extracted from the cancer cells after treatment with the plant extract and subjected to two-dimensional electrophoresis for protein separation. Further, the proteins that were differentially regulated among the samples which were treated and non-treated were selected and processed further for protein identification using a tandem mass spectrometry approach.Results: Using these strategies, we identified ۱۶ potential candidates which were showing remarkable changes in treated samples. All the candidates were analyzed further for gene ontology analysis, and it was observed that all proteins were involved in multiple pathways pertaining to the carcinogenesis process. Specifically, apoptotic pathway proteins including BAD, BIK, BID, CASP۸, MCL۱, BCL۲, and BAK۱ were highly impacted by treatment with D. falcata plant extract. All these protein hits were further taken for validation experiments using RT PCR analysis. Conclusion: Initiation of these apoptotic proteins by D. falcata plant extract treatment in breast cancer cells shows a positive direction toward nature-based alternative medicine.

۲D-Gel Electrophoresis, Breast cancer cell line, Dendropthae falcate, Mass spectrometry, Medicinal plant, Proteomics