Evelyn Komaratih - Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Clarisa Finanda - Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Wimbo Sasono - Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Nurwasis Nurwasis - Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Citra Dewi Maharani - Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Kautsar Abiyoga - Department of Ophthalmology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Djoko Legowo - Department of Pathology Anatomy, Faculty of Veterinary, Universitas Airlangga, Surabaya, Indonesia
Hari Basuki Notobroto - Department of Epidemiology, Biostatistics, Population Studies and Health Promotion, Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia
Willy Sandhika - Department of Pathology Anatomy, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

Patients with open globe injury (OGI) are higher risk of developing secondary retinal detachment. This study examines the effectiveness and safety of a single intravitreal of triamcinolone acetonide or bevacizumab to suppress the expression of VEGF and TNF-α in OGI rabbit eye. OGI is generated by inflicting a ۵-mm posterior ocular lesion with blade no. ۱۱ and ۶-mm inferior the limbus in the supratemporal quadrant. Intravitreal injection with triamcinolone acetonide (TA) ۴ mg/۰.۱ mL or bevacizumab ۱.۲۵ mg/۵۰ µL ۳ days (T+۳ and B+۳ groups) and ۷ days (T+۷ and B+۷ groups) after OGI (n = ۶). Another OGI group without treatment served as the positive control (CtrP) while five left eyes were randomly selected from the treated group to serve as the negative control (CtrN). Pretreatment and decapitation, a comprehensive anterior segment, posterior segment, and intraocular pressure (IOP) examination was conducted on days ۳, ۷, and ۲۱. On day ۲۱, the eyes were enucleated, and VEGF and TNF-α expression were assessed by immunohistochemistry. VEGF and TNF-α overexpression were observed in rabbit eyes after OGI (۹.۶۴ ± ۱,۶۴۶, p = ۰.۰۰۰۲ and ۱۰.۴۴ ± ۱,۳۸۱, and p = ۰.۰۵۵۳, respectively). Treatments significantly reduced these pro-inflammatory proteins compared with eyes in the OGI group. Posthoc Tukey’s multiple comparison test showed that TA gave better downregulation than that of bevacizumab. Clinical examination found no significant rise of IOP at the end of the study. The current study exhibits TA intravitreal injection after OGI significantly attenuated the overexpression of pro-inflammatory protein with a good safety profile.

Open globe injury Proliferative vitreoretinopathy Triamcinolone acetonide Bevacizumab Good health Well, being