What is the preferred method for diagnosing cryptosporidiosis in sheep - Modified Ziehl–Neelsen or auramine O staining?

Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
View: 47

متن کامل این Paper منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل Paper (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_JZD-7-3_006

تاریخ نمایه سازی: 29 شهریور 1402

Abstract:

Cryptosporidium is an intestinal apicomplexan protozoon that causes cryptosporidiosis, a diarrheal disease affecting gastrointestinal tract of a wide range of vertebrates, including humans, livestock, wild animals, and birds. In the present study, Cryptosporidium spp. infection was diagnosed in sheep using two staining methods: Modified Ziehl–Neelsen (MZN) and auramine O (AO) staining. A total of ۲۶۰ fecal samples were collected from sheep. All the samples were subjected to the formalin-ether concentration technique. The fecal smears were stained using the MZN (acid-fast stain) and AO (fluorescent stain) staining methods and then observed under a light and a fluorescent microscope, respectively. Cohen's kappa was used to check the significant agreement between the two diagnostic methods. According to the results, there was substantial agreement between the two methods. However, since there are many fluorescent artifacts in sheep feces, the detection rapidity of Cryptosporidium spp. in sheep feces by MZN staining is higher than that of AO staining. Therefore, it is suggested to use MZN staining for diagnosing cryptosporidiosis in sheep flocks.

Authors

Mehdi Sohrabi

Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran

Hassan Nayebzadeh

Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran

Hamidreza Shokrani

Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran

Karin Gohardehi

Deaprtment of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran

Zahra Amini Farsani

Bayesian Imaging and Spatial Statistics Group, Institute for Statistics, Ludwig-Maximilians University of Munich, Ludwigstreet ۳۳, ۸۰۵۳۹ Munich, Germany & Department of Statistics, School of science, Lorestan University, Khorramabad,

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • ReferencesAl-alousi T. I., & Mahmood O. I. Detection of Cryptosporidium ...
  • Bukhari Z., & Smith H. Effect of three concentration techniques ...
  • Caccio S. M. New methods for the diagnosis of Cryptosporidium ...
  • Carey C. M., Lee H., Trevors J. T. Biology, persistence ...
  • Casemore D. P., Armstrong M., Sands R. L. Laboratory diagnosis ...
  • Dubey J. P., Speer C. A., Fayer R. ۱۹۹۰, Cryptosporidiosis ...
  • Fayer R., Morgan U., Upton S. J. Epidemiology of Cryptosporidium: ...
  • Huang D. B., White A. C. An updated review on ...
  • Insulander M., Lebbad M., Stenström T. A., Svenungsson B. An ...
  • Khurana S., Sharma P., Sharma A., Malla N. Evaluation of ...
  • Quadros R. M., Marques S. M., Amendoeira C. R., Souza ...
  • Ranjbar-Bahadori Sh., Sangsefidi H., Shemshadi B., Kashefinejad M. Cryptosporidiosis and ...
  • Sim, J., Wright, C. C. The kappa statistic in reliability ...
  • نمایش کامل مراجع