D. Lamas - Technology, Valorization and Innovation of Fishery Products Program of the National Institute for Fisheries Research and Development (INIDEP), Institute of Marine and Coastal Research (IIMyC), National Council for Scientific and Technical Research (CONICE
A. Massa - Technology, Valorization and Innovation of Fishery Products Program of the National Institute for Fisheries Research and Development (INIDEP), Institute of Marine and Coastal Research (IIMyC), National Council for Scientific and Technical Research (CONICE

Nowadays, the need to simultaneously achieve the valorization and recovery of fishing waste is critical for addressing environmental challenges and promoting resource sustainability. Fishing waste hosts enzymes that hold the potential for extraction and utilization in various industries. In this sense, the aim of this work was to extract and purify enzymes from the gastrointestinal tract of Myliobatis goodei using low-cost processes. The proteolytic and lipolytic activities of the extracted enzymes were also investigated. In addition, the detergent compatibility of the purified enzyme extract was evaluated to determine its potential application as an additive in laundry soaps. The crude extract was obtained by homogenization of ۱۰۰g gastrointestinal tract in buffer Tris-HCl (pH=۸.۰). Then, it was precipitated with ammonium sulfate and purified by filtration. Finally, it was subjected to centrifugation at ۱۰,۰۰۰ ×g for ۳۰ min at ۴°C in an ultrafilters membrane with a ۱۰۰ kDa cut-off. This purification protocol showed good performance for proteases and lipases as their activity was recovered at each step. The proteolytic activity was assayed using azocasein as substrate at pH ۸.۰ and ۴۰°C, while trypsin activity was analyzed against the specific substrate benzoyl-arginine-p-nitroanilide. The lipolytic activity was carried out by p-nitrophenol production through the reaction of p-nitrophenylacetate dissolved in ۲-propanol at pH ۷.۰ and ۳۷°C. The compatibility of the enzyme activity with some commercial detergents was evaluated. Additionally, proteolytic and lipolytic activities were tested on spiruline and soybean oil stains and showed a strong ability to remove them from cotton fabrics. Due to its promising properties, the purified enzymes isolated from the gastrointestinal tract of M. goodei may be considered as a potential effective active ingredient for its use in the detergents industry.

Circular economy, Fish waste, Myliobatis goodei, Proteases, Lipases, Detergent compatibility