Maryam rezaie, - Department of Microbiology, Shiraz Unit, Apadana Institute of Higher Education, Shiraz, Fars Province,Iran
Aida Fijani, - Department of Microbiology, Shiraz Unit, Apadana Institute of Higher Education, Shiraz, Fars Province,Iran
Kowsar Kazemi, - Department of Microbiology, Shiraz Unit, Apadana Institute of Higher Education, Shiraz, Fars Province,Iran
Nazanin Ebadi, - Department of Microbiology, Shiraz Unit, Apadana Institute of Higher Education, Shiraz, Fars Province,Iran
Sahar Abdolahi, - Department of Microbiology, Shiraz Unit, Apadana Institute of Higher Education, Shiraz, Fars Province,Iran
mozhgan Sedigh-Arddakani - Department of Microbiology, Shiraz Unit, Apadana Institute of Higher Education, Shiraz, Fars Province,Iran

Background: MicroRNAs (miRNAs) are endogenous, ۱۸-۲۲ nucleotide noncoding RNA molecules. Human cytomegalovirus (HCMV) is a ubiquitous and highly specific herpes virus, encode miRNAs, which increases gradually in the presence of infection. One of the important viral miRNAs is known as HCMV-miR-UL۱۱۲ which plays a role in the establishment and maintenance of viral latency. Objectives: The current study aimed to evaluate the expression levels of HCMV-miRUL-۱۱۲, in active and inactive transplant patient groups in comparison to healthy individuals. Materials & Methods: Total RNA was isolated from samples of ۶۰ organ transplant patients and ۳۰ controls, in-house SYBR green Real-time PCR protocols was performed for miRNA and gene. Results: The expression level of UL-۱۱۲ gene was significantly higher in the active HCMV infected patients (p= ۰.۰۰۱). The miR-UL۱۱۲ expression level significantly increased in the inactive HCMV infected patient (p<۰.۰۰۱). Conclusion: Increase of the miR-UL۱۱۲ expression level in inactive HCMV infected transplant patients introduce the potential role of the miR-UL-۱۱۲ as a biomarker index of HCMV latent stage that should be confirmed in future completed studies.

Human Cytomegalovirus, miRNA, Transplantation, miR-UL۱۱۲