Yousof Naghiaee - Department of Biochemistry and Molecular Biology, School of Medicine, ShahidSadoughi University of Medical Sciences, Yazd, Iran.
Javad Mohiti Ardakani - ۱. Department of Biochemistry and Molecular Biology, School of Medicine, ShahidSadoughi University of Medical Sciences, Yazd, Iran. ۲. Diabetes Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Fatemeh Pourrajab - Department of Biochemistry and Molecular Biology, School of Medicine, ShahidSadoughi University of Medical Sciences, Yazd, Iran.Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Masoud Rahmanian - Department of Endocrinology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Objective: Insulin resistance (IR) is the major cause in Type ۲ diabetes mellitus (T۲DM). Expression of some miRNAs can be changed in response to a drug treatment for IR, and used as the biomarker in IR. This study set out to determine the effect of cinnamon extract (cinnamaldehyde) on some miRNAs expression in IR adipocytes. Materials and Methods: In this In-vitro study the ۳T۳L۱ cells were expanded in Dulbecco’s modified Eagle’s medium (DMEM), differentiated into adipocytes phenotype and insulin resistant with high glucose medium, then the cells were treated with cinnamaldehyde. To determine of the miRNAs profiling in ۳T۳L۱ adipocytes, insulin-resistant adipocytes and treated insulin-resistant adipocytes quantitative real-time PCR method was performed. Results: IR adipocytes exhibited a significantly increase in miRs ۲۹a, ۲۲۳ and ۳۲۰ expression, and decrease in miR۲۶-b expression in compare to the normal adipocytes (P-value<۰.۰۰۱ and P-value= ۰.۰۲۴ respectively). However in response to cinnamaldehyde in IR adipocytes, expression of miRs ۲۹a, ۲۲۳ and ۳۲۰ were down-regulated while expression of miR۲۶-b was up-regulated neared it to the normal level (P-value= ۰.۰۰۳ and P-value= ۰.۰۰۲ respectively). Conclusion: IR changes expression of intended miRs, so that cinnamaldehyde treatment helps to improve and normalize the changes. Cinnamon as the herbal product can be helpful for IR particular in adipose tissue.

Insulin resistance, Diabetes, miRNAs, Cinnamon, Cinnamaldehyde, Adipocytes