Metallophosphoesterases of Chlamydomonas reinhardtii and Analyses of Their Transcription Levels Under Phosphate Deficiency
عنوان مقاله: Metallophosphoesterases of Chlamydomonas reinhardtii and Analyses of Their Transcription Levels Under Phosphate Deficiency
شناسه ملی مقاله: JR_JASTMO-25-2_004
منتشر شده در در سال 1401
شناسه ملی مقاله: JR_JASTMO-25-2_004
منتشر شده در در سال 1401
مشخصات نویسندگان مقاله:
G. Hatice Havutcu - Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye.
M. Aksoy - Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye.
خلاصه مقاله:
G. Hatice Havutcu - Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye.
M. Aksoy - Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye.
Phosphorous (P) is an important macroelement for all organisms. However, there is a finite amount of P on Earth, therefore, new enzymes and technologies are needed for better P use in agriculture. Metallophosphoesterases are a large group of evolutionarily related proteins that are important in biotechnology. We found fourteen putative Metallophosphoesterase (MPA) genes in the genome of Chlamydomonas reinhardtii. Our RT-PCR analyses showed that some of these genes were constitutively expressed, and some were upregulated under phosphate deficiency. These results and bioinformatic analyses suggest that two of the genes (MPA۱۱ and MPA۱۳) are transcribed in high levels and the putative polypeptides are predicted to be secreted to extracellular space, making them ideal to be used in biotechnological applications. Phylogenetic analyses show that MPA۱۱ and MPA۱۳ are related to known phytases from plant species, suggesting MPA۱۱ and MPA۱۳ might have specific phytase activity. In light of these results, we discuss the potential of C. reinhardtii as a phytase producing organism for agricultural and industrial use.
کلمات کلیدی: Phosphate nutrition, Phytase, Phytic acid, Phosphate.
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1815998/