Mohammad Arjmand - Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
Abdolah Mirzaei - Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mohammad Reza Jafarzadeh Shirazi - Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
Amin Tamadon - Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Mohammad Saied Salehi - Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
Mehdi Saeb - Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mohammad Reza Namavar - Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences and Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Mohammad Reza Zandi - Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
Hojatollah Shahheidari - Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
Sara Moradi - Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran

The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-۱۰ injection on the secretion of luteinizing hormone (LH) in female anestrous goats. In the first study, progesterone (P۴) concentration in ۱۰ goats in summer (n = ۶) and winter (n = ۴) were measured every other day. Moreover, in summer group, a male teaser goat was left in the herd on days of sampling for one hour. Goats with P۴ concentration ≥۱ ng mL-۱, at least two consecutive measurements, were considered with luteal activity. In the second study, the anestrous phase was confirmed by P۴ measurement ۲۰ and ۱۰ days before the kisspeptin injection in five female Abadeh ecotype goats (۴ to ۵ years old). The goats were given a single IV injection of saline (۲ mL) as control group and the same goats (۱ hr after the last blood sampling) were given kisspeptin (۱ μg kg-۱) as treatment group. The blood samples were collected at –۶۰, –۴۰, –۲۰ and ۰ min (before injection), and ۱۰, ۲۰, ۳۰, ۴۰, ۵۰, ۶۰, ۸۰, ۱۰۰, ۱۲۰ and ۱۴۰ min after the injection and LH concentration were measured. A single IV injection of ۱ µg kg-۱ of kisspeptin-۱۰ did not stimulate the release of LH in female anestrous goats. In summer, in the presence of teaser goat, luteal activity was seen in all goats. In the absence of male goat in winter, some goats showed luteal activity and others showed anestrus.

Anestrus, Goat, Kisspeptin, Luteal activity, Luteinizing hormone