Hadi Eslami - Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Rooz Ali Batavani - Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Siamak Asr‎i-Rezaei - Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Rahim Hobbenaghi - Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

The present study investigated the effects of E. coli lipopolysaccharide (LPS) induced mastitis model in rat on the activity of antioxidant enzyme systems. To achieve this purpose, E. coli LPS were infused into the mammary gland of ۱۲ rats ۷۲ hr postpartum and compared with ۱۲ rats in control group infused intramammary placebo sterile pyrogene – free, physiological saline. The antioxidant activities of the enzymes, superoxide dismutase, glutathione peroxidase, and catalase together with ‎malondialdehyde (MDA) level were assayed in blood serum, milk and mammary tissue. Results obtained showed that, the antioxidant enzyme activities in milk, blood serum and mammary tissue were significantly decreased while the level of MDA, the indicator of lipid peroxidation were significantly increased following intramammary inoculation of LPS compared to the control animals. Histopathological examination also revealed the infiltration of inflammatory cells in mammary tissue and disruption of acinar structure and acinar lumina in mastitic rats. The results indicated that LPS-induced ‎mastitis could alter antioxidant enzymes and increase lipid peroxidation.

Antioxidant enzymes, Lipopolysaccharide, malondialdehyde, Mastitis, Rat