Saeed Rasoulinezhad - Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
Mohammad Hassan Bozorgmehrifard - Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
Hossein Hosseini - Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
Nariman Sheikhi - Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
Saeed Charkhkar - Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran

Mycoplasma gallisepticum (MG) is economically important pathogen of poultry causes airsacculitis and frequently infraorbital sinusitis in turkeys. Infections may remain without clinical signs, but they can make birds susceptible to secondary infections.This study was carried out for molecular detection and phylogenetic analysis of MG infections in commercial and backyard turkey flocks in some parts of Iran. A total number of ۶۰۰ swab samples were collected from ۱۸ commercial and ۳۱ backyard turkey flocks. The PCR technique was performed for detecting ۱۶S rRNA gene in the samples. Positive sample were subjected for sequencing of mgc۲ gene. The results showed that ۴۸.۳۸% of backyard and ۱۶.۶۶% of commercial farms were positive for MG. These findings suggested the presence of MG in the commercial and backyard turkeys’ farms of Iran. The molecular analysis indicated high sequence similarity between some Iranian turkeys isolates with Indian and Pakistanian MG isolates. Furthermore, substitutions of MG nucleic acids and correlated amino acids sequences may lead to some antigenic modifications.

Iran, Molecular detection, Mycoplasma gallisepticum, Phylogenetic, Turkey