Farhad Bahraminia - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Seyed Reza Emadi - Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Mohammad Emaneini - Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Nima Farzaneh - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Mehrnaz Rad - Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Babak Khoramian - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

The macrolides appear to have considerable effects for treatment of bovine mastitis because of excellent diffusion into the mammary gland, long half-life, low protein binding, high intracellular concentration and lipid solubility. Acquired resistance to macrolides in Staphylococcus aureus is primarily related to target-site modification through acquisition of an erm gene. In the present study the prevalence of both phenotypic and genotypic tylosin resistance in S. aureus isolates (n = ۱۰۳) from subclinical mastitis in nine dairy farms belonging to three different province of Iran were investigated. Overall, ermA, ermB and ermC was found in ۷.۸۰%, ۳۲.۰۰%, and ۲۰.۴۰% of S.aureus isolates, respectively. A very high percent of isolates (۵۶.۹۰%) were resistant to tylosin. MIC۹۰ and MIC۵۰ values were ۶۴ and ۳۲ µg mL-۱, respectively. Most of tylosin resistant isolates did not harbour any erm gene but ermB was dominant gene among ۵۸ tylosin resistant isolates of S. aureus. In overall, tylosin resistance was prevalent in S. aureus isolates obtained from bovine mastitis in Iran.

Bovine mastitis, erm genes, Macrolides, Staphylococcus aureus, Tylosin resistance