Vahid Akbarinejad - Young Researchers and Elites Club, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Parviz Tajik - Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Mansoureh Movahedin - Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Reza Youssefi - Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Testosterone is believed to play a significant role in spermatogenesis, but its contribution to the process of spermatogenesis is not completely understood. Given that extracellular matrix (ECM) facilitates differentiation of spermatogonial stem cells (SSCs) during culture, the present study was conducted to elucidate whether testosterone contribute to the permissive effect of ECM on SSCs differentiation. In experiment ۱, testosterone production was measured in testicular cells cultured for ۱۲ days on ECM or plastic (control). In experiment ۲, testosterone production was assessed in testicular cells cultured on ECM or plastic (control) and exposed to different concentrations of hCG. In experiment ۳, the gene expression of factors involved in testosterone production was analyzed. Testosterone concentration was lower in ECM than in the control group in experiment ۱ (p < ۰.۰۵). In experiment ۲, testosterone concentration was increased in response to hCG in both groups but cells cultured on ECM were more responsive to hCG than those cultured on plastic (p < ۰.۰۵). In the experiment ۳, qRT-PCR revealed the inhibitory effect of ECM on the gene expression of steroidogenic acute regulatory protein (StAR) (p < ۰.۰۵). Nevertheless, the expression of LH receptor was greater in ECM-exposed than in unexposed cells (p < ۰.۰۵). In conclusion, the present study showed that inhibiting the expression of StAR, ECM could lower testosterone production by Leydig cells during in vitro culture. In addition, the results indicated that ECM could augment the responsiveness of Leydig cells to hCG through stimulating the expression of LH receptor.

Bovine testicular cells, Extracellular matrix, Testosterone