Ozlem Şahan Yapicier - Bacteriological Diagnostic Laboratory, Veterinary Control Central Research Institute, Republic of Turkiye Ministry of Agriculture and Forestry, Ankara, Turkiye
Dilek Ozturk - Department of Microbiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkiye
Mehmet Kaya - Department of Microbiology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkiye

The purpose of this study was to investigate the tetracycline resistance in Trueperella pyogenes isolates from bovine samples in Burdur, Turkiye, and assess ۱۶ tetracycline-resistance genes distribution among the isolates. Forty-nine T. pyogenes isolates were phenotypically characterized for anti-microbial resistance to doxycycline, oxytetracycline and tetracycline by disc diffusion method. Presence of tetracycline genes of T. pyogenes was investigated by multiplex and singleplex polymerase chain reaction. Our results indicated that ۸۷.۸۰% and ۴۲.۸۶% of the isolates were resistant to tetracycline and oxytetracycline, respectively, and the rate of resistance to doxycycline was ۶.۱۲%. Total of ۲۱ (۴۲.۸۵%) were carrying tetracycline-resistance genes and tet(A) was present in ۱۲ (۲۴.۴۹%) isolates; whereas, the tet(W) gene was identified in ۹ (۱۸.۳۷%) and ۲ (۴.۰۸%) of the isolates carried both tet(A) and tet(W), respectively. The study indicated antibiotic resistance patterns of tetracycline agents and links to the tet-genes among T. pyogenes were detected. It makes it worthwhile that this is the first report for detection of tet(A) gene in T. pyogenes.

Cattle, Mastitis, tetracycline resistance genes, Trueperella pyogenes