Zahra Bakhtiary - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎
Rasoul Shahrooz - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎
Rahim Hobbenaghi - Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎
Saeed Azizi - Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎
Farhad Soltanalinejad - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎
Ali Baradar Khoshfetrat - Department of Chemical Engineering, Sahand University of Technology, Tabriz, Iran‎

Skeletal muscle atrophy induced by denervation is one of the common disorders in traumatic nerve injuries. The aim of this study was the evaluation of histomorphometrical changes of extensor digitorum longus muscle after denervation and its regeneration by tissue engineering. Ninety adult male Wistar rats were randomly divided into six main groups (n = ۱۵) in three time periods (۲, ۴ and ۸ weeks; n = ۵). Control group was treated without surgery, in transection (Tr) group left sciatic nerve was transected, in scaffold (S) group only collagen gel scaffold was used, in mast cell (MC) group mast cells were used, mesenchymal stem cell (MSC) group was treated with mesenchymal stem cells and in MC+MSC group, mast cells along with mesenchymal stem cells were used. In the cellular groups, the scaffold and cells were mixed and placed in the transected nerve gap. The average diameter of muscle fibers, ratio of the muscle fibers nuclei to the fibrocytes nuclei (mn/fn), ratio of the muscle fibers nuclei number to the muscle fibers number (mn/mf), the average ratio of blood vessels to muscle fibers number (v/mf) and muscles weight in Tr group were the lowest compared to the other groups; but, in cellular and S groups, amelioration was observed according to the time period. However, in MC+MSC group, there were the highest ameliorative results. This study revealed that simultaneous use of MCs and MSCs mixed with collagen gel scaffold can be considered as a suitable approach to improve denervated skeletal muscle atrophy associated with sciatic nerve injury.

Extensor digitorum longus muscle, Mast cell, Mesenchymal stem cell, Neural tissue engineering, Rat