Jamshid Razmyar - Department of Poultry Diseases, Faculty of veterinary medicine, University of Tehran,Tehran, Iran
Mahdis Ghavidel - PhD student of Bacteriology, Department of Microbiology, Mashhad University of Medical Sciences, Mashhad, Iran
Hamideh Salari Sedigh - Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Genus Brachyspira,as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of Brachyspira spp. for the first time in Iran and rapid identification of Brachyspira spp. in dogs by a new designment of a species-specific primer set for B. canis. One hundred fifty-one fecal samples were obtained from dogs by rectal swab. Twenty dogs suffered from diarrhea and ۱۳۱ of them were healthy. In ۹.۲۷% (۱۴/۱۵۱) of samples, spirochaetes were detected on primary cultures by weak hemolysis and positive Gram staining and then Brachyspira genus was confirmed by NADH oxidase (nox) gene via polymerase chain reaction. Among ۱۴ isolates, twelve isolates were B. canis, one isolate was B. intermedia and another one was non-typeable. From ۱۲ B. canis, only eight isolates were detected by designed specific primers. Ten Brachyspira spp. were isolated from dogs ≤ ۱ year old (۱۰/۶۷, ۱۴.۹۲%) and ۴ isolates were from > ۱ year old dogs (۴/۸۴, ۴.۷۶%). The isolation rates from healthy and diarrheic dogs were (۱۲/۱۳۱, ۹.۱۶%) and (۲/۲۰, ۱۰.۰۰%), respectively. A statistically significant association was observed between the presence of Brachyspira spp. and the age under one year. Based on our findings, the nox gene in B. canis might have more sequence variability compared to other Brachyspira spp.

Brachyspiracanis, Dogs, Iran, nox gene, PCR