Neda Abedpour - Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Mojdeh Salehnia - Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Nassim Ghorbanmehr - Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

Lysophosphatidic acid (LPA) known as a serum-derived growth factor, is involved in several cell physiological functions in the female reproductive system including: oocyte maturation, in vitro fertilization and embryo implantation by its transmembrane G protein-coupled receptors. The aim of the present study was to examine the effect of LPA on in vitro follicular development of mouse ovarian tissue. Neonatal mouse ovarian tissues were cultured in five different concentrations of LPA (۰, ۵, ۱۰, ۲۰ and ۴۰ µM). The developmental competence and the function of cultured ovarian tissue were assessed by morphological study using hematoxylin and eosin staining and hormonal analysis. The expression of LPA receptor (LPAR ۱-۴) genes were analyzed by real-time RT-PCR. The proportion of preantral follicles and the level of E۲ hormone were significantly higher in the ۲۰ µM LPA-treated group than those in the other treatment groups. There was a significant difference in the expression of LPAR ۱-۴ genes in ۲۰ µM LPA treated group in comparison with ۰ µM LPA (control group) treated and non-cultured groups. In addition, the expression of LPAR۱ gene was higher than other receptor genes in all studied groups. In conclusion supplementation of the media with ۲۰ µM LPA, could improve the survival and developmental potential of follicles and it had positive effects on cell function and stimulation of E۲ synthesis in mouse whole ovarian tissues.

‎ In vitro culture, ‎ Lysophosphatidic acid, ‎ Lysophosphatidic acid receptor, ‎ Mouse, ‎ Ovary