Mohammad Afkhami-Ardakani - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Shapour Hasanzadeh - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Rasoul Shahrooz - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Norouz Delirezh - Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Hassan Malekinejad - Department of Basic Scinces, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran

Cyclophosphamide (CP) is known to reduce fertility. The protective effects of Spirulina plantesis (SP) against CP-induced testicular toxicity were investigated. Male Wistar rats were categorized into eight groups (n = ۷). Four groups of rats were administered CP at a dose of ۵ mg in ۵ mL distilled water kg-۱ per day orally. Two of these groups were received SP (۵۰۰ and ۱۰۰۰ mg kg-۱ per day) orally after CP administration. One of these groups was also received vitamin E (۱۰۰ mg kg-۱ per day) intraperitoneally. A vehicle treated control group, two SP control groups (۵۰۰ and ۱۰۰۰ mg kg-۱ per day) and a vitamin E control group were also included. Body and testes weights, sperm count, serum levels of glutathione peroxidase (GPx), malondialdehyde (MDA), histological and histomorphometric alternations in testes were investigated after four weeks. The CP-treated group exhibited significant decreases in the body and testes weights and spermatogenic activities. Several histological alterations were observed in this group. The CP treatment caused a significant reduction in sperm count, in serum level of GPx, as well increased serum concentration of MDA. The SP co-administration caused an increase in GPx serum level, a decrease in MDA serum level and improvements in histological and histomorphometric alternations. Vitamin E co-treatment showed partial recovery in above-mentioned parameters. These results suggest that SP due to a reduction in oxidative stress has more effective protection against CP-induced reproductive damages in rat than vitamin E.

Cyclophosphamide, ‎ Male reproductive toxicity, ‎ Oxidative stress, ‎ Rat, ‎ Spirulina plantesis ‎