Jun-Ting Liu - Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China
Yan-Hui Chen - Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China
Yi-Feng Pei - Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China
Qian Yu - Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China
Ruth Afumba - Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China
Hao Dong - Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China

Gosling plague caused by goose parvovirus (GPV), a highly infectious septic disease with high mortality, has caused substantial loss in the waterfowl industry. A method for the rapid detection of GPV is needed. In this study, we isolated the virus strain of GPV in May ۲۰۲۰ and applied it to the loop-mediated isothermal amplification (LAMP) assay. We designed five sets of primers for the goose parvovirus VP۳ gene by LAMP. The GV-۱ primer set was selected to detect GPV sensitively and rapidly. LAMP was more sensitive compared to PCR. In addition, the LAMP method could complete detection within ۶۰ min which was faster than the PCR assay. The LAMP provided a convenient and effective experimental method for detection of GPV for inspection and quarantine departments and health care units in China, and it is expected to become a simple and routine detection method, especially suitable for goose farms.

goose parvovirus, Gosling plague, Loop-mediated isothermal amplification