Iman Yousefian - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Ahmad Zare-Shahneh - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
Hamid Kohram - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

The effects of MitoTEMPO, a mitochondria-targeted antioxidant, and its non-targeted parent, TEMPO, on bovine oocyte maturation competence have not been determined so far. Hence, our study was aimed to investigate the effects of supplementing maturation medium with different concentrations of MitoTEMPO (۰.۰۰, ۰.۱۰, ۱.۰۰ and ۱۰.۰۰ µM) or TEMPO (۰.۰۰, ۵.۰۰, ۱۰.۰۰ and ۱۵.۰۰ mM) on in vitro maturation (IVM) and fertilization (IVF) of bovine oocytes. The oocytes after IVM and IVF were evaluated for the signs of nuclear maturation and normal fertilization. The average number of spermatozoa penetrated per oocyte and the level of intracellular reactive oxygen species (ROS) were also evaluated. The results showed that percentages of bovine oocytes reached the metaphase II stage of meiosis were significantly higher in the ۱.۰۰ µM MitoTEMPO group compared to the control group (without antioxidant supplementation). The normal fertilization rate also tended to be greater in this group than the control group. In comparison with the control group, the medium supplementation with ۱.۰۰ µM MitoTEMPO led to a significant decrease in the intracellular ROS level. The average number of spermatozoa penetrated per oocyte was not significantly different among the antioxidant-treated and the non-treated groups. The TEMPO addition to the maturation medium affected neither the rate of maturation/fertilization nor the level of intracellular ROS in bovine oocytes. Based on these results, we concluded that MitoTEMPO at a concentration of ۱.۰۰ µM had beneficial effects on the quality and fertilization potential of bovine oocytes.

Antioxidant, ‎ Cattle, ‎ Fertilization, ‎ Maturation, ‎ Reactive oxygen species