The Role of miR-۱۴۳, miR-۱۴۵, and miR-۵۹۰ in Expression Levels of CD۴۴ and Vascular Endothelial Cadherin in Oral Squamous Cell Carcinoma

Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_MISJ-10-3_004

تاریخ نمایه سازی: 25 آبان 1402

Abstract:

Background: Oral squamous cell carcinoma (OSCC) is the most common head and neck cancer with a continuing rise of incidence in developing countries. Despite the improvement in the clinical outcome of OSCC, the overall ۵-year survival rate of patients is still disappointing. MicroRNAs (miRNAs) regulate gene expression in the post-transcriptional stage by targeting mRNAs. Advances in knowledge of the pathogenesis and molecular events lead to the improvement of OSCC treatments. Materials and Methods: Human oral epidermoid carcinoma cells (KB), HGF۱, and HEK ۲۹۳T cell line were cultured. Lentiviral vectors were constructed and Western Blot analysis was performed. Then, immunocytochemistry staining was performed by using CD۴۴ and vascular endothelial cadherin (VE-Cadherin) antibodies. All data were analyzed using the REST ۲۰۰۹ software. P values of ≤۰.۰۵ were considered statistically significant. Results: MiR-۱۴۳, miR-۱۴۵, and miR ۵۹۰ were down-regulated in the oral cancer cell line. Following transfection of Lv miR ۱۴۳, Lv-miR-۱۴۵, and Lv-miR-۵۹۰, the expression of CD۴۴ was markedly decreased in KB cells. In addition, transfection of miR-۱۴۳ and miR- ۵۹۰ mimics into the oral cancer cell line significantly decreased the expresion level of VE-Cadherin; however, transfection of miR-۱۴۵ mimic had no significant effect on the expression of VE-Cadherin. Western blot analysis and immunocytochemistry staining confirmed the results. Conclusion: This study revealed that miR-۱۴۳, miR-۱۴۵, and miR-۵۹۰ negatively regulate CD۴۴ and VE-Cadherin (except miR-۱۴۵) expression, which might play a crucial role in the induction of cancer stem cells proliferation and angiogenesis in OSCC cells. The knowledge about the involved factors may provide new insights for the clinical use of miR- ۱۴۳, mR-۱۴۵, and miR-۵۹۰ in the treatment of patients with OSCC.

Authors

Soussan Irani

Dental Research Center, Oral Pathology Department, Dental Faculty, Hamadan University of Medical Sciences, Hamadan, Iran

Gelareh Shokri

Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran