Reza Farsiabi - Department of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, Iran
Iraj Khodadadi - Department of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, Iran
Jamshid Karimi - Department of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, Iran
Gholamreza Shafiee - Department of Clinical Biochemistry, Medicine School, Hamadan University of Medical Sciences, Hamadan, Iran

Background: Thymoquinone (TQ), an active part of Nigella sativa, has been reported as an anticancer agent. This study aimed to evaluate different anticancer effects of TQ on oxidative stress markers and Peroxiredoxin ۴ (P۴) in lung cancer A۵۴۹ cell line. Method: In this experimental study, we used TQ concentrations to treat lung A۵۴۹ cells and determined cell viability by the ۳-(۴,۵-dimethylthiazol-۲-yl)-۲,۵- diphenyltetrazolium bromide (MTT) assay at ۱۲, ۲۴, and ۴۸ h times. The IC۵۰ concentration of TQ was found with MTT assay. We studied the total antioxidant capacity (TAC) and total oxidant status (TOS) using the manual assay, and analyzed catalase (CAT), superoxide dismutases (SOD), and glutathione peroxidase (GPx) activity using the enzyme-linked immunoassay (ELISA) kits. Moreover, the concentration of peroxiredoxin-۴ (PRXD۴) was measured with the ELISA Kit. Results: The IC۵۰ of TQ for A۵۴۹ cells was calculated to be ۴۰ μM concentration for ۲۴ h of incubation. TAC index significantly decreased in the treated cells compared with the controls (P = ۰.۰۵), whereas TOS and PRXD۴ levels showed a significant increase (P = ۰.۰۵). Additionally, the results showed that the CAT, SOD, and GPX activity enzymes significantly decreased in ۲۰, ۴۰, and ۶۰ μM TQ in comparison with the control cells (P = ۰.۰۵). Conclusion: TQ has significant inhibitory effects on A۵۴۹ cells and could be utilized in novel therapy not only for lung cancer, but also for other tumors.

Thymoquinone, Oxidative stress, Peroxiredoxin ۴, Lung Neoplasms