Manizhe Jozpanahi - Department of Infectious Disease, Zanjan University of Medical Sciences, Zanjan, Iran
Rojin Ahmadi - School of Medicine, Islamic Azad University Medical Branch of Tehran, Iran
Afsaneh Ayaz - Department of Infectious Disease, Ardabil University of Medical Sciences, Ardabil, Iran
Fakhri Haghi - Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

Objective: A tropical disease of zoonotic origin, Leptospirosis is a public health concern, particularly in developing countries. This present investigation sought to determine the seroprevalence of Leptospira in healthy people referred to Shahid Motahari Laboratory, Zanjan, Iran.Methods: ۱۸۱ healthy people referred to Shahid Motahari laboratory in Zanjan, Iran, were included in the present study. Serum samples were collected from each participant, whose demographic information was gathered using a questionnaire. After being stored -۲۰ °C, the samples were analyzed for Anti-Leptospira IgG antibodies using NovaLisa Leptospira IgG ELISA" kit, the results of which were reported as positive, negative, or equivocal.Results: Out of ۱۸۱ serum samples, ۴۳ (۲۳.۷%) samples were positive, ۳۶ (۱۹.۹%) were equivocal, and ۱۰۲ (۵۶.۴%) were negative for anti-Leptospira IgG. The average age of the participants was ۴۰.۶ years old. No significant difference was shown between age and gender with anti-Leptospira IgG. IgG levels were not found to be correlated significantly with the rural residence of the participants, as well as the other demographic characteristics of participants.Conclusion: The results of this investigation contribute to the understanding of the epidemiological aspects of Leptospirosis in the region and underscore the importance of continued surveillance and awareness efforts.

Leptospirosis, Seroprevalence, ELISA