J. Wojtacka - Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ۱۴ Oczapowskiego str, ۱۰-۷۱۸ Olsztyn, Poland.
B. Wysok - Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ۱۴ Oczapowskiego str, ۱۰-۷۱۸ Olsztyn, Poland.
A. Kaba&scaroninskienė - Department of Food Safety and Quality, Faculty of Veterinary Medicine, Lithuanian University of Health Sciences, ۱۸ Tilzes str, LT-۴۷۱۸۱ Kaunas, Lithuania.
A. Wiszniewska-Łaszczych - Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ۱۴ Oczapowskiego str, ۱۰-۷۱۸ Olsztyn, Poland.
M. Gomółka-Pawlicka - Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ۱۴ Oczapowskiego str, ۱۰-۷۱۸ Olsztyn, Poland.
J. Szteyn - Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ۱۴ Oczapowskiego str, ۱۰-۷۱۸ Olsztyn, Poland.
M. Malakauskas - Department of Food Safety and Quality, Faculty of Veterinary Medicine, Lithuanian University of Health Sciences, ۱۸ Tilzes str, LT-۴۷۱۸۱ Kaunas, Lithuania.
A. Migowska-Calik - Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, ۱۴ Oczapowskiego str, ۱۰-۷۱۸ Olsztyn, Poland.

The aim of the study was the analysis of Lithuanian honeys for the presence of the spores of Clostridium botulinum. The analyses were performed on multifloral, honeydew, nectar-honeydew, rape, buckwheat, heather and linden honey bought in direct sale. Prior to culture on solid media the samples were prepared by supernatant filtration method and cultured in parallel in Cooked Meat Medium (CMM) and Tripticase Peptone Glucose Yeast (TPGY) enrichment broths. Bacteria of typical phenotypic features were obtained by culture method from ۴۸ honey samples. Identification of Clostridium botulinum types A, B, E and F was performed with the use of multiplex-PCR method. The prevalence analysis showed ۳۰ (۶۰%) samples contaminated with Clostrdium botulinum spores. The major serotype detected was type A: ۲۰ (۶۶.۶%) positive honey samples; while type B was found in ۷ samples (۲۳.۳%), type E in ۴ samples (۱۳.۳%) and type F in ۳ (۱۰%) positive honey samples. Contamination with more than one type of C. botulinum spores was observed in ۳ honey samples.

Botulism, Contamination, C. botulinum spores, Cooked meat medium, Multiplex-PCR, TPGY