S. Afshari-Behbahanizadeh - Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, Tehran, Islamic Republic of Iran.
Gh. A. Akbari - Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, Tehran, Islamic Republic of Iran.
M. Shahbazi - Department of Molecular Physiology, Agriculture Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Islamic Republic of Iran.
I. Alahdadi - Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, Tehran, Islamic Republic of Iran.
L. Farahani - Young Researchers and Elite club, Islamic Azad University, Arak Branch, Arak, Islamic Republic of Iran.
S. A. Tabatabaee - Agriculture and Natural Resources Research Center of Yazd, Yazd, Islamic Republic of Iran.
M. Ganji - Department of Agronomy, Plant Breeding and Biotechnology, University of Agricultural and Natural Resources, Mazandaran, Islamic Republic of Iran.

Drought stress is the major limitation for crop yield, which depending on the time of occurrence, could decrease the number of grain as well as their weight. Barley (Hordeum vulgare L.) is one of the tolerant cereals that its grain components have an important role in human and animal nutrition; however, physical and biochemical properties of grains affected through drought stress are still poorly understood. In this study, barley genotypes (n= ۶) with different levels of drought tolerance were studied in a ۲-year field experiment under well-watered and terminal drought stress conditions. In order to measure physical properties of grains, digital images were taken and some morphological features were obtained by using Image Analysis Toolbox of MATLAB software. Biochemical properties of grains were also measured. Results proved that size, weight and also quality of the grains were significantly affected by drought stress (P< ۰.۰۱). Grain starch content decreased and protein content increased under drought stress at anthesis stage in all genotypes, but drought-sensitive genotypes interestingly had more percentage increase in protein content. Furthermore, genotypes varied in total sugar, sucrose, glucose and fructose content. Drought stress affected grain size and finally ۱,۰۰۰-grain weight of barley genotypes by reducing area and minor axis length of grains. Correlations between ۱,۰۰۰-grain weight and minor axis length, grain area, starch and sucrose content were significant (P< ۰.۰۱). These results emphasized in both conditions that size-dependent features of grain particularly minor axis length and area may be serving as useful traits for estimation of ۱,۰۰۰-grain weight and biochemical properties in barley.

Grain size, Hordeum vulgare L, Image analysis, Protein, Starch