Yirgashewa Wuhibe - Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite Ethiopia
Getaw Zewdu - Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite Ethiopia
Getu W/Yohannes - Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite Ethiopia
Degisew Mengistu - Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite Ethiopia

Withania somnifera (L.) Dunal is frequently used as traditional medicine for the treatment of numerous diseases. This study was aimed to assess bioactive compounds, antimicrobial activities and antioxidant studies on different part of W. somnifera. The plant parts were shaded dried and grinded to powders for biological activities. The ethanol, methanol and chloroform extracts were tested for antimicrobial activities. The extracts were subjected to a test of their antimicrobial properties by paper disc diffusion method. Minimum inhibitory concentrations (MICs) of the plant extracts against selected pathogens were also assessed using the agar dilution method. The highest potential was observed from methanol extract of the stem bark and ethanol extract of the leafagainst Staphylococcus aurous with zone of inhibition ۱۵.۰۲ and ۱۳.۲۲ mm, respectively.Alkaloids, steroids, saponins, tannins, reducing sugars, phlobatanins, flavonoids, terpenoids, cardiac glycosides, anthraquinone, emodins and coumarins are result of phytochemical assessment. Strong antioxidant effect of free radical scavenging activity was recorded at ۱۰۰µg/ml concentration for ethanol stem and root, methanol leaf extracts (۷۲.۲۲%, ۸۰.۸۴%, ۷۴.۲۴%) respectively. Chloramphenicol and DMSO were used as positive and negative controls. W. somnifera could show powerful antimicrobial and antioxidant properties.

Antibacterial, Antioxidant, In-vitro, MIC, Paper disc diffusion, Withania somnifera