Mohammad Hossein Mirjalili - Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
Fatemeh Amiri - Mahan Golsay Company, Yazd, Iran
Seyed Mostafa Goldansaz - Mahan Golsay Company, Yazd, Iran

Because of the special situation of medicinal plants and their natural product on human health, researchers are interested to discover useful constituents. The present research aims to discover the level of variation in oil of Ziziphora clinopodioides subsp. rigida (Boiss) Rech. f. at full flowering stage in Yazd province. All samples were approved in herbarium of Yazd University and dried out in shade and laboratory situation. Then ۵۰ grams of each sample were powdered. In order to extract the oil of each community, the dried materials were subjected by hydro-distillation method (clavenger types). Resulted oils were dried by anhydrous sodium sulfate and kept in tightly closed vials at ۴ °C before chemical analyses. The GC-FID and GC–MS analyses identified ۲۹ compounds among which pulegone (۱۸.۱-۵۱.۴%), piperitenone (۱۰.۳-۲۳.۳%), and ۱,۸-Cineole (۸.۶-۱۵.۶%) were the major component in all populations. It is indicated that the present result is similar to the previous study. In addition, Damgahan and Tezerjan had the highest amount of essential oil and pulegone in all studied populations, respectively.

Composition, Variation, Ziziphora, Iran