Patricia Garcia-Palencia - School of Biological Sciencies, Universidad Autonoma de Nuevo Leon, Pedro de Alba s/n, Ciudad Universitaria San Nicolas de Los Garza, Mexico
Catalina Rivas-Morales - School of Biological Sciencies, Universidad Autonoma de Nuevo Leon, Pedro de Alba s/n, Ciudad Universitaria San Nicolas de Los Garza, Mexico
Azucena Oranday-Cardenas - School of Biological Sciencies, Universidad Autonoma de Nuevo Leon, Pedro de Alba s/n, Ciudad Universitaria San Nicolas de Los Garza, Mexico
Myriam De La Garza-Ramos - School of Dentistry,“Dr. Jose Eleuterio Gonzalez” University Hospital, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico
Catalina Leos-Rivas - School of Biological Sciencies, Universidad Autonoma de Nuevo Leon, Pedro de Alba s/n, Ciudad Universitaria San Nicolas de Los Garza, Mexico
Ernesto Torres López - School of Medicine,“Dr. Jose Eleuterio Gonzalez” University Hospital, Universidad Autonoma de Nuevo Leon, Monterrey, Mexico

Periodontal diseases are characterized by inflammation and destruction of tooth-supporting tissues. There is interest in anti-inflammatory substances of plant origin because of their advantage in relation to classic anti-inflammatory drugs. The cytotoxic and anti-inflammatory effect of extracts of Scopulophila parryi and Psacalium decompositum (A.Gray) H.Rob. & Brettell were evaluated with the ۳-[۴,۵-dimethylthiazole-۲-yl]-۲,۵-diphenyltetrazolium (MTT) method and their pro-inflammatory cytokine profile was determined in a human peripheral blood monocyte culture model. The plant was collected and identified and the methanol extract was prepared by maceration. Cytotoxicity was evaluated in vitro with the MTT (۳-[۴,۵-dimethylthiazole-۲-yl]-۲,۵-diphenyltetrazolium) assay in two cell lines, VERO and Detroit ۵۵۱ human fibroblasts. The anti-inflammatory activity of human peripheral blood monocytes and gene expression of the proinflammatory cytokines, IL-۱, IL-۶ and IL-۸, were evaluated The extract of P. decompositum was less toxic on the VERO and Detroit ۵۵۱ cell lines with a DL۵۰ of ۷۷.۳۰ μg/mL and the S. parryi extract had ۱۵۰.۵۸ μg/mL. Both extracts significantly reduced expression of IL-۱β, IL-۶, and IL-۸ at ۳۰۰ μg/mL for S. parryi and at ۴۰۰ μg/mL for P. decompositum. The extract of P. decompositum was less toxic for both cell lines and the two methanol extracts are good alternatives as immunomodulators of proinflammatory cytokines.

Cytokines, Toxicity, cell lines, periodontal