Farhad Niknejad - Infectious Disease Research Science, Golestan University of Medical Sciences, Gorgan, Iran
Mojtaba Nabili - Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
Roshanak Daie Ghazvini - Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Maryam Moazeni - Invasive Fungi Research Centre, Department of Medical Mycology & Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Background and Purpose: Microorganism-based synthesis of nanostructures has recently been noted as a green method for the sustainable development of nanotechnology. Nowadays, there have been numerous studies on the emerging resistant pathogenic bacteria and fungal isolates, the probable inability of bacteria and fungi to develop resistance against silver nanoparticles’ (SNPs) antibacterial, antifungal, antiviral and, particularly antibacterial activities. In this study, we aim to use the yeast Saccharomyces cerevisiae model for synthesis of SNPs and to investigate its antifungal activity against some isolates of Candida albicans. Materials and Methods: A standard strain of S. cerevisiae was grown in liquid medium containing mineral salt then, it was exposed to ۲ mM AgNO۳. The reduction of Ag+ ions to metal nanoparticles was virtually investigated by tracing the color of the solution, which turned into reddish-brown after ۷۲ hours. Further characterization of synthesized SNPs was performed afterwards. In addition, antifungal activity of synthesized SNPs was evaluated against fluconazolesusceptible and fluconazole-resistant isolates of Candida albicans. Results: The UV-vis spectra demonstrated a broad peak centering at ۴۱۰ nm, which is associated with the particle sizes much less than ۷۰ nm. The results of TEM demonstrated fairly uniform, spherical and small in size particles with almost ۸۳.۶% ranging between ۵ and ۲۰ nm. The zeta potential of SNPs was negative and equal to -۲۵.۰ (minus ۲۵) mv suggesting that there was not much aggregation. Silver nanoparticles synthesized by S. cerevisiae, showed antifungal activity against fluconazole-susceptible and fluconazole-resistant Candida albicans isolates, and exhibited MIC۹۰ values of ۲ and ۴ &mug/ml, respectively. Conclusion: The yeast S. cerevisiae model demonstrated the potential for extracellular synthesis of fairly monodisperse silver nanoparticles

Biosynthesis, Extracellular, Saccharomyces Cerevisiae, Silver nanoparticles