Zohreh Karimi - Department of Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Mohammad Shokrzadeh - Department of Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Ali Abbasi - Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran. Department of Community Medicine, Sari Branch, Islamic Azad University, Sari, Iran.
Vahid Sharifi - Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.

A Simple and fast QuEChERS method has been applied for extraction of imidacloprid from human biological samples. Imidacloprid the was extracted from blood and liver of a deceased body admitted to the legal Medicine of Mazandaran. Factors affecting the extraction procedure such as type of the organic solvent and its volume, amount of salt, amount of sorbent, and pH were examined and optimized for the maximum recovery. The appropriate condition for extraction of imidacloprid was as follows: sample pH=۵, ۳mL of acetonitrile, ۰.۱ g of NaCl, and ۰.۴ g of MgSO۴. The maximum recovery of imidacloprid at these conditions was ۹۶%. The LOD and LOQ were ۰.۰۲ and ۰.۰۶ ppm, respectively. Finally, the blood and liver samples were extracted under the appropriate condition and determined using HPLC which is equipped with a photodiode array detector. The amounts of Imidacloprid in blood and liver samples were ۱۱ and ۱.۸ ppm, respectively.

Biological samples, Forensic Toxicology, HPLC, Imidacloprid, QuEChERS