Mohammad Shokrzadeh - Department of Pharmacology and Toxicology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Parham Mortazavi - Student Research Committee, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Elhame Karimi - Student Research Committee, Ramsar International Branch, Mazandaran University of Medical Sciences, Ramsar, Iran.
Behnam NasirOghli - Student Research Committee, Ramsar International Branch, Mazandaran University of Medical Sciences, Ramsar, Iran.
Shaghayegh Shokrzadeh - Student Research Committee, Ramsar International Branch, Mazandaran University of Medical Sciences, Ramsar, Iran.
Farzaneh Motafeghi - School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

Introduction: Various studies have shown that vincristine and permethrin have genetic toxicity on the body's normal cells. Due to the widespread use of these drugs, preventing their toxicity is essential; Therefore, in this study, the protective effects of vitamin C and melatonin on the genetic toxicity induced by vincristine and permethrin in peripheral blood lymphocytes were investigated. Materials and Methods: The protective effects of vitamin C and melatonin (doses of ۵۰, ۱۰۰, and ۲۰۰ µm) on the toxicity of vincristine and permethrin induced by micronucleus test on peripheral blood lymphocytes were evaluated, and in statistical tests P<۰.۰۵ as the significant level was considered. Results: According to the results, vincristine and permethrin caused genetic disorder by ۲۸.۸۰±۱.۹۲ and ۳۴±۱.۵۸ micronucleus, respectively (P<۰.۰۰۰۱). However, by exposing vitamin c and melatonin with permethrin at concentrations of ۱۰۰ μM and ۲۰۰ μM, the number of micronuclei was significantly decreased by ۲۴.۸۰±۲.۹۱, ۱۸.۰۰±۱.۵۸ (Vit C) and ۲۲.۲۰±۳.۳۴, ۱۵.۴۰±۱.۱۴ (melatonin) respectively. In comparison, exposure of these two substances with vincristine in similar concentrations reduced the micronucleus by ۱۶.۶۰±۲.۰۷, ۱۰.۸۰±۰.۸۳ (Vit C) and ۱۳.۰۰±۱.۵۸, ۶.۴۰±۱.۱۴ (melatonin), respectively. Conclusion: As the results of this study showed, permethrin and vincristine both caused genetic toxicity. Melatonin can protect against DNA damage by purifying reactive oxygen species or stimulating the DNA repair system. Vitamin C plays an essential protective role in many toxic reactions of the body. Both antioxidants have been shown to reduce the genetic toxicity of permethrin and vincristine.

Genotoxicity, Melatonin, Permethrin, Vincristine, Vitamin C