Mosbah Asma - Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Mentouri Constantine ۱, Constantine, Algeria- Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas Setif ۱, Algeria
Khither Hanane - Laboratory of Applied Biochemistry, Faculty of Natural and Life Sciences, University Ferhat Abbas Setif ۱, Algeria
Mosbah Camelia - Laboratory of Natural Substances, Bioactive Molecules and Biotechnological Applications, LarbiBen M’hidi University, Oum El Bouagui, Algeria
Slimani Abdelkader - Unit of the Valorization of Natural Resources, Bioactive Molecules and Physicochemical and Biological Analysis, Faculty of Exact Sciences, University Mentouri Constantine ۱, Algeria

Introduction: Nigella sativa L. is a widely used medicinal plant throughout the world. The low toxic effects and low price of this plant make it an excellent treatment choice for many diseases. The present study aims to investigate the hepatoprotective effect of N. sativa L. total oil (TO) and its neutral lipid fraction (NLF) via the estimation of circulating xanthine oxidoreductase (XOR) level and anti-XOR antibodies titer. Methods: Antiradical activities of TO and NLF, in vitro, were carried out using three reactive oxygen species (ROS), superoxide anion, hydroxyl radical, and hydrogen peroxide. In vivo study was conducted to determine the possible protective effects of TO and NLF against ethanol-induced hepatotoxicity in rats feeding Lieber-DeCarli liquid diet in both sera and liver homogenate. Before conducting the hepatoprotective effects, we assessed the toxicity of our extracts using the same animal model. The administrated doses of ۴۰۰ mg/kg for TO and ۳۰۰ mg/kg for NLF showed no toxic effects. Results: ELISA assay indicated a significant increase (P < ۰.۰۰۱) in the level of XOR and the titer of anti-XOR antibodies in rats treated with ethanol compared to the control group. After treatment with TO and NLF, the titer of anti-XOR antibodies and the level of XOR decreased significantly compared to the control group. Conclusion: XOR plays an important role in alcohol liver pathologies as a major source of free radicals. In addition, TO and NLF have significant potential as liver protective agents and might be utilized as new antioxidant therapeutics.