Rajesh R Patil - Department of Pharmacology, Sinhgad Institute of Pharmaceutical Sciences, Kusgaon (Bk.), Lonavala, Pune ۴۱۱ ۴۰۱, (MS) India
Manoj K Aswar - Department of Pharmacology, Rajarshi Shahu College of Pharmacy, Buldana-۴۴۳۰۰۱(MS), India
Satish Bhise - Department of Pharmacology, Sinhgad Institute of Pharmaceutical Sciences, Kusgaon (Bk.), Lonavala, Pune ۴۱۱ ۴۰۱, (MS) India
Suresh R Naik - Department of Pharmacology, Sinhgad Institute of Pharmaceutical Sciences, Kusgaon (Bk.), Lonavala, Pune ۴۱۱ ۴۰۱, (MS) India

Introduction: Natural polyphenols, rutin, endogenous polycations, and polyamines (spermine and spermidine) are reported to have beneficial effects on hyperlipidemia, obesity, and cardiovascular disease (CVD). The present study attempts to evaluate the combined effects of polyamines or rutin and simvastatin on hyperlipidemic rats. Methods: Wistar rats were maintained on a high-fat diet (HFD) for ۶۰ days. The HFD rats were administered from the ۳۱st day onward with polyamines (PAs), rutin, and simvastatin for the next ۳۰ days. The body weight, serum lipid profile, biomarkers, liver cholesterol, triglycerides, hydroxymethylglutaryl-CoA (HMG-CoA) reductase activity, antioxidants, and marker enzymes in HFD rats were estimated along with the liver histoarchitecture of the rats. Results: The experimental findings demonstrated abnormal alterations in body weight, serum lipid profile, hepatic lipid, and HMG-CoA reductase activity, hepatic antioxidants, serum marker enzymes, blood glucose, total protein, and histoarchitecture of the liver in HFD rats. The aforementioned treatment elicited a significant reduction in the biochemical parameters, biomarkers, and the evaluated enzymes in the present study. Furthermore, an improvement in the histoarchitecture of the liver was observed. Conclusion: The experimental results point out the positive role of antioxidant polyamines along with rutin on different parameters in HFD rats. Thus the combinatorial effect of polyamines and rutin with simvastatin (۵ mg/kg) was found to be greater than simvastatin (۱۰ mg/kg) alone. The enhancement in the anti-hyperlipidemic effect of simvastatin may be due to the intrinsic antioxidant property of polyamines and rutin.

Dyslipidemia, Spermine, Spermidine, Antioxidants, Combination therapy