Syaiful Bahri - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Lampung, Jalan Soemanatri Brojonegoro No.۱ Bandar Lampung ۳۵۱۴۵, Lampung, Indonesia
Annisa Elcentia Fajarwati - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Lampung, Jalan Soemanatri Brojonegoro No.۱ Bandar Lampung ۳۵۱۴۵, Lampung, Indonesia
Andi Setiawan - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Lampung, Jalan Soemanatri Brojonegoro No.۱ Bandar Lampung ۳۵۱۴۵, Lampung, Indonesia
John Hendri - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Lampung, Jalan Soemanatri Brojonegoro No.۱ Bandar Lampung ۳۵۱۴۵, Lampung, Indonesia
Suripto Dwi Yuwono - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Lampung, Jalan Soemanatri Brojonegoro No.۱ Bandar Lampung ۳۵۱۴۵, Lampung, Indonesia
Yuli Ambarwati - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Lampung, Jalan Soemanatri Brojonegoro No.۱ Bandar Lampung ۳۵۱۴۵, Lampung, Indonesia
Rahadian Zainul - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, West Sumatra, Indonesia

In this study, we explore a steroid compound from the endophytic of the mangrove (Avicennia marina). Penicillium sp. is one of the microbes endophytic isolated from the mangrove and induced in the chitin medium by the so-called "cultivation". The cultivation in the medium is a strategy to enhance secondary metabolites production. In addition, the steroid compound of the secondary metabolites can be extracted and isolated from the purified fraction, followed by analysis and characterization to validate the target compound. Using the acquired ۱۴MA۱۵ fraction as a representative sample, we found that it presents a peak in the UV spectrum at a wavelength of ۲۰۶ nm from the carbonyl group. The stretching vibration of C=O group in the IR spectrum is also confirmed at ۱۷۱۲ cm-۱. Furthermore, other stretching vibrations from the O-H group, C-O group, and –C–H group are detected at ۳۴۱۰ cm-۱, ۱۰۵۵ cm-۱, and ۱۴۶۳ - ۷۲۳ cm-۱, respectively. Therefore, it is concluded that the steroid compound proposed has a molecular formula of C۲۶H۴۰O۲ according to MS fragmentation analysis.

antibacterial, Avicennia marina, endophytic, Penicillium sp, Steroid