Anita Dalal - Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
Naresh Kakker - Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
Deepika Chaudhary - Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
Aman Kumar - Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
Narender Maan - Department of Animal Nutrition College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
Sushila Maan - Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

World Organization for Animal Health has listed bluetongue (BT) under notifiable diseases. The BT is an arboviral infectious disease of domestic and wild ruminants caused by the bluetongue virus (BTV). Southern states of India had remained the point of attention for BT since first presence in ۱۹۶۴ in Maharashtra. Recently, northern states of India have also been reported positive for BTV in small ruminants. The present study reported the dual infection of BTV serotypes, BTV-۱۲ and -۱۶ in sheep population from Sirsa district of Haryana in the year ۲۰۱۶. After detection and serotyping with Seg-۲ specific real time polymerase chain reaction (PCR), the Seg-۲ and Seg-۶ of BTV were PCR amplified and sequenced. On phylogenetic analysis it was detected to be clustered in nucleotype G and nucleotype B specific for BTV-۱۲ and BTV-۱۶, respectively. This was the first report of BTV-۱۶ from Haryana. The results signified the co-infection of two different serotypes in an animal from a single outbreak.

Bluetongue virus, Dual Infection, Goat, Molecular diagnosis, real time RT-PCR