Hamed Khodayari - PhD Student / Department of Biology, University of Isfahan, Isfahan, Iran
Hojjatollah Saeidi - Assistant Professor / Department of Biology, University of Isfahan, Isfahan, Iran
Mohammad Reza Rahiminejad - Professor / Department of Biology, University of Isfahan, Isfahan, Iran
Takao Komatsuda - Professor / Crop Genome Research Unit, National Institute of Agrobiological Sciences (NIAS), ۲-۱-۲ Kannondai, Tsukuba, Ibaraki, ۳۰۵-۸۶۰۲, Japan

Limited numbers of microsatellite markers are available for genetic characterization of Hordeum bulbosum which comprises the secondary genepool of cultivated barely. The objective of this study was to evaluate the transferability of microsatellite markers from H. vulgare to H. bulbosum and a preliminary evaluation of their polymorphism. From ninety-three pairs barley SSR primer tested for transferability, all of them  amplified DNA segments in H. vulgare (۱۱ accessions) and۴۸ pairs (۵۱.۶۱%) were transferable to the H. bulbosum (۵ accessions) with high level of polymorphism. Twenty-two (۲۳.۶۵%) SSR markers showed transferability to H. murinum used as outgroup. A total of ۵۴۶ alleles were detected by ۴۸ transferred primer pairs in all accessions. The number of alleles per locus ranged from ۳ to ۱۳ with an average of ۱۱.۳۷۵ alleles per locus. The PICvalues were ranged from ۰.۱۶۱ to ۰.۶۲۱ with an average of ۰.۴۷۷. The value of PIC in H. vulgare (average PIC = ۰.۶۳۹) was significantly higher than H. bulbosum (average PIC = ۰.۳۱۶). In dendrogram generated based on SSR data accessions were divided into groups related to their taxonomic classifications, indicating the efficiency of barley SSRs for phylogenetic analyses in H genome containing species in the genus Hordeum. Based on the results of this study, it can be suggested that the cross species transferable barley SSRs are valuable molecular tools, for genetic diversity analyses in the H. bulbosum for which limited number of microsatellite markers are available. This study provided a set of efficient SSR markers from publicly available barley microsatellite markers for the genetic characterization of H. bulbosum.

Microsatellites, Genetic diversity, Breeding, Transferability, H genome, Barley, germplasm