Isolation and Optimization of Compound Bacteria for Wax Content and Particle Size

Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JPSTR-9-4_004

تاریخ نمایه سازی: 29 آذر 1402

Abstract:

For removing wax and reducing viscosity of wax, microbial technology can effectively be used, and thereby, by using this technology, the flow properties of crude oil are improved. In this study, optimization of compound bacteria isolated from oil-contaminated soil and its effect on wax content and particle size are investigated. Moreover, experimental results show that the optimized compound bacteria have the highest cell density value under the optimal conditions. After #۱۱ and #۱۲ compound bacteria are added, the wax content reduces from ۲۱.۴۶% to ۸.۳%, and the wax removal rate becomes ۶۱.۳۲%. In addition, the polarizing microscope analysis demonstrates that the paraffin is degraded with the bacteria, and its wax crystal micro-structure is changed with the bacteria. Moreover, the focused beam reflectance measurement (FBRM) shows that the proportion of particles of large diameter is significantly decreased, and that of small ones is increased. Consequently, the flow properties of waxy crude oil are improved. The viscosity changes at different temperatures after treatment with bacteria. Finally, it is found out that at the temperature which is equal to ۴۰ to ۴۵ ºC, the rate of viscosity reduction is higher than ۶۰%. Therefore, the paraffinic hydrocarbons of crude oil can be degraded with compound bacteria, and their rheological properties are improved.

Authors

WeiQiang Wang

College of Petroleum Engineering,Liaoning shihua University,Fushun ۱۱۳۰۰۱,Liaoning,China

Jing Cui

College of Petroleum Engineering,Liaoning shihua University,Fushun ۱۱۳۰۰۱,Liaoning,China

ShangShu Wu

College of Petroleum Engineering,Liaoning shihua University,Fushun ۱۱۳۰۰۱,Liaoning,China

Yun Cheng

College of Petroleum Engineering,Liaoning shihua University,Fushun ۱۱۳۰۰۱,Liaoning,China

GuoFu Wang

College of Petroleum Engineering,Liaoning shihua University,Fushun ۱۱۳۰۰۱,Liaoning,China

HaiJuan Zhang

College of Petroleum Engineering,Liaoning shihua University,Fushun ۱۱۳۰۰۱,Liaoning,China

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