K. Kukreti - Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh
L. Pandey - St. aloysius College, Jabalpur (MP), India
M. Das - Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, ۲۵۰۰۰۱, India
A. Rastogi - Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, ۲۵۰۰۰۱, India
R. Dubey - Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, ۲۵۰۰۰۱, India
P. Sharma - Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, ۲۵۰۰۰۱, India

Canine ehrlichiosis is a very important emerging disease in India. This study is the first attempt screening a large number of canines in India for the detection of canine monocytic ehrlichiosis. In the present study, ۵۱۰ blood samples of dogs were screened for the presence of Ehrlichia canis and other variants of Anaplasmataceae family by serological and molecular methods.Out of the ۵۱۰ serum samples, ۲۹۳ (۵۷.۵%) cases were found positive for the presence of E. canis antibodies through enzyme-linked immunosorbent assay (ELISA). Furthermore, and ۴۵ (۸.۸%) and ۱ (۰.۲%) specimens were positive for E. canis and A. platys, respectively, based on the polymerase chain reaction (PCR). In the clinical samples of E. canis, the minimum detection limit for PCR was۹ ng. In the immunofluorescence assay (IFA), the positive blood samples showed comparable results with those obtained from the commercially available dot ELISA kit (giving equivalent IFA titer). The results of sequencing were compared with other reported isolates in various regions of the world, and a phylogenetic relationship was established. The ۱۶S rRNA region that was amplified and sequenced for E. canis and A. platys was highly conserved and so was another Vir B۹ region.

Ehrlichiosis canis, Anaplasma platys, Polymerase Chain Reaction, ELISA, India