Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
عنوان مقاله: Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
شناسه ملی مقاله: JR_ARCHRAZI-59-2_008
منتشر شده در در سال 1384
شناسه ملی مقاله: JR_ARCHRAZI-59-2_008
منتشر شده در در سال 1384
مشخصات نویسندگان مقاله:
H. Ghadakchi
H. Dadras
S.A. Pourbakhsh
S.M.H. Hosseini
خلاصه مقاله:
H. Ghadakchi
H. Dadras
S.A. Pourbakhsh
S.M.H. Hosseini
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR ۸۰۱ strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined ۰.۱μg/ml, ۱:۳۰۰۰ and ۱:۱۰۰, respectively. The optical densities (OD) were compared with a commercial ELISA kit. Also, the results of ELISA were compared to hemagglutination inhibition (HI) test. The correlation coefficient of the results of both ELISAs and HI was significant (P
کلمات کلیدی: Infectious bronchitis, ELISA, Antibody, Massachusetts serotype
صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/1869040/