Mohammad Karami - Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Seyed Sajad Ale-Nabi - Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran
Anahita Nosrati - Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Atefeh Naimifar - Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran

The aim of present study was to investigate the potential antioxidant and lung protective activities of Sambucus ebulus (SE) against toxicity induced by gamma irradiation. Hydroalcoholic extract of SE (۲۰, ۵۰ and ۱۰۰ mg/kg) was studied for its lung protective activity. Phenol and flavonoid contents of SE were determined. Male C۵۷ mice were divided into ten groups with five mice per group. Only the first and second groups (as negative control) received intraperitoneally normal saline fluid. Groups ۳ to ۵ received only SE extract at doses of ۲۰ mg/kg, ۵۰ mg/kg and ۱۰۰ mg/kg intraperitoneally; three groups were repeatedly injected for ۱۵ days as chronic group. Groups ۶ to ۸ received a single-dose of gamma irradiation just ۲ hours before irradiation as acute group. The ninth and tenth groups (as positive control) received only gamma rays. Animal was exposed whole-body to ۶ Gy gamma radiation. After irradiation, tissue sections of lung parenchyma were examined by light microscope for any histopathologic changes. SE at doses ۵۰ and ۱۰۰ mg/kg improved markedly histopathological changes induced by gamma irradiation in lung. Lung protective effect of SE could be due to attention of lipid peroxidation. Our study demonstrated that SE as a natural product has a protective effect against lung toxicity induced by   gamma irradiation in animal.

Sambucus ebulusi, Gamma irradiation, Histopathology changes, Antioxidant, Lung Protective