zohreh majlesi - Plant Biotechnology Group, Sana Institute of Higher Education, Sari, Iran
Moazzameh Ramezani - Plant Physiology Group, Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
Mahyar Gerami - Plant Physiology Group, Sana Institute of Higher Education, Sari, Iran

Stevia rebaudiana B. extract as a low-calorie sweetener has gained worldwide approval. Synthesized nanoparticles by plant have been used in different programs such as medical, pharmaceutical and agricultural fields. Here, we report the increase of the main glycosides of stevia plant with silver nanoparticles (AgNPs) treatment. This fact has stimulated research on the analysis and comparison of different concentrations of commercial and synthesized AgNPs on the glycosides of stevia plant by high-performance liquid chromatography (HPLC). After AgNPs synthesis, spectrum absorption of extracts was analyzed in different ranges from ۳۰۰ to ۷۰۰ nm and the maximum peak was observed at ۴۲۰ nm. Transmission electron microscopy (TEM) micrograph of synthesized AgNPs from Stevia rebaudiana extract showed spherical shape with the size of ۲۵ nm. A comparison between quantities of glycoside by HPLC under both treatments of AgNP exhibited that synthesized AgNPs were more effective than commercial AgNPs on stevioside and then rebaudioside. On the other hand, results showed that increasing AgNP concentration in both treatments will lead to glycoside content enhancement. Overall, significant changes were observed in the glycosides in plants under synthesized and commercial AgNP treatment that could be used to create plants with higher quality of glycosides for pharmaceutical approaches.

Silver nanoparticle, green synthesis silver nanoparticle, plant extracts, Stevia rebaudiana